Testing The Statistics On The Distribution Of Ccr5∆32;
...population
Abstract:
Homozygous individuals who possess a 32 base pair deletion (∆32) in the CC-chemokine receptor CCR-5 gene have been shown to have increased resistance to HIV-1. The present experiment was undertaken in order to study the presence of the deletion in a sample population of 12 Caucasian individuals. Based on previous literature it is hypothesized that 2.4 out of the twelve individuals will exhibit the mutation. The DNA was collected, amplified using PCR, separated using gel electrophoresis, and visualized under UV light. The results of the experiment were inconclusive therefore the hypothesis cold neither be supported nor rejected. It is believed that the DNA was lost either through purification or the digest.
Introduction:
Members of the chemokine receptor gene family recently have been identified as the co-receptors which allow entry of human immunodeficiency virus type 1 (HIV-1) into human CD4-positive cells.1 The chemokine receptors are members of a superfamily of seven transmembrane loops and transduce their signals through heterotrimeric G-proteins.2 These receptors can be classified as either CXC- (or [alpha]-) or CC- (or [beta]-) chemokine receptors, according to the primary structure of their natural agonists.3 As can be seen below in figure 1, there are three types of chemokine receptors that are grouped as specific (bind only one known ligand - e.g., CXCR1/IL8RA and CXCR4/fusin/LESTR), shared (CXCR2/IL8RB, CXCR3, CCCR1-CCCR5), promiscuous (bind to many chemokine ligands of either CXC or CC types - e.g. Duffy blood group antigen), and viral (shared receptors that have been transduced into viral genomes during evolution - herpes saimiri virus and cytomegalovirus).2
The CCR-5 gene which is located in the p21.3 region of the human chromosome three along with other CC-chemokine receptor genes1, has been shown as the...
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